NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of … NettetHoechst 33342 Solution (20 mM) Emission 497 nm Form Liquid Product Line Pierce™ Shipping Condition Room Temperature Product Type Stain SubCellular Localization Upon receipt store at 4°C and protect from light. Hoechst 33342, Trihydrochloride, Trihydrate … This stain binds into the minor groove of DNA and exhibits distinct fluorescence … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes SYTO dyes are live cell, cell-permeant nucleic acid stains that exhibit bright … • Emission wavelength: 518 nm • Extinction coefficient: > 70,000 M-1 cm-1 • CAS #: … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
Hoechst 33342/PI Double Staining Protocol Creative Bioarray
Nettet11. sep. 2024 · The table below (Table 2) contains data on the peak excitation and emission wavelengths of over 75 selected fluorochromes along with suggested applications and excitation filter cube type. The selection of a wideband, superwide band or narrow band filter cube will depend on the condition of the specimen and observation … Nettet460-490 nm. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence … thicket\u0027s lf
Enumeration of marine microbial organisms by flow cytometry …
Nettet16. mar. 2024 · The two best wavelengths would be those that produced the maximum signal on the excitation and emission spectra. That will lead to the most sensitivity and … NettetHowever, the closer the excitation wavelength is to 495 nm, the more fluorescence will be produced. This optimal wavelength is called the excitation peak. Similarly, the light produced by fluorochromes has a range of wavelengths. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately … NettetExcitation Wavelength Range 350 nm Dye Type Cell-Permeant For Use With (Application) Fluorescent labeling For Use With (Equipment) Fluorescence Microscope Quantity 100 mg Detection Method Fluorescence Description Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg Emission 461 nm Form Solid Product Line Hoechst Shipping Condition Room … thicket\\u0027s le